Recent Publications
2024
Mitja M Zdouc et al. "MIBiG 4.0: advancing biosynthetic gene cluster curation through global collaboration." Nucleic Acids Research, 2024 Dec 09; Database Issue 1-13.
M. Simone, M. Iorio, P. Monciardini, et al. "The molecules gateway: a homogeneous, searchable database of 150k annotated molecules from actinomycetes." The Journal of Natural Products, 2024 October 25; 87/Issue 11: 2615-2628.
Daniele Ghezzi et al. ” Ancient and remote quartzite caves as a novel source of culturable microbes with biotechnological potential ” Microbiological Research 2024 Sept. Volume 286; 127793.
2023
Vind K, Brunati C, Simone M, Sosio M, Donadio S, Iorio M. ” Megalochelin, a Tridecapeptide Siderophore from a Talented Streptomycete ” ACS Chem. Biol. 2023 March 15; 18(4):861-874.
2022
Iorio M, Gentile A, Brunati C, Tocchetti A, Landini P, Maffioli SI, Donadio S, Sosio M. “Allopeptimicins: unique antibacterial metabolites generated by hybrid PKS-NRPS, with original self-defense mechanism in Actinoallomurus”. RSC Adv. 2022 Jun 6;12(26):16640-16655.
Miethke M, Pieroni M, Weber T, Brönstrup M, et al., “Towards the sustainable discovery and development of new antibiotics”. Nat Rev Chem. 2021;5(10):726-749.
Vind K, Maffioli S, Fernandez Ciruelos B, Waschulin V, Brunati C, Simone M, Sosio M, Donadio S.” N-Acetyl-Cysteinylated Streptophenazines from Streptomyces.” J Nat Prod. 2022 May 27;85(5):1239-1247.
Zhang M, Kong L, Gong R, Iorio M, Donadio S, Deng Z, Sosio M, Chen W.”Biosynthesis of C-nucleoside antibiotics in actinobacteria: recent advances and future developments”. Microb Cell Fact. 2022 Jan 4;21(1):2.
Waschulin V, Borsetto C, James R, Newsham KK, Donadio S, Corre C, Wellington E. “ Biosynthetic potential of uncultured Antarctic soil bacteria revealed through long-read metagenomic sequencing.” ISME J. 2022 Jan;16(1):101-111.
2021
Breitling R, Avbelj M, Bilyk O, Carratore FD, Filisetti A, Hanko EKR, Iorio M, Redondo RP, Reyes F, Rudden M, Severi E, Slemc L, Schmidt K, Whittall DR, Donadio S, García AR, Genilloud O, Kosec G, De Lucrezia D, Petković H, Thomas G, Takano E. "Synthetic biology approaches to actinomycete strain improvement" FEMS Microbiol Lett. (2021) 368:fnab060. doi: 10.1093/femsle/fnab060.
Del Carratore F, Iorio M, Pérez-Bonilla M, Schmidt K, Pérez-Redondo R, Sosio M, Macdonald SJ, Gyulev IS, Tsigkinopoulou A, Thomas GH, Genilloud O, Rodríguez-García A, Donadio S, Breitling R, Takano E. "Multi-omics Study of Planobispora rosea, Producer of the Thiopeptide Antibiotic GE2270A". mSystems. (2021) 6(3):e0034121. doi: 10.1128/mSystems.00341-21.
Iorio, M., Davatgarbenam, S., Serina, S., Criscenzo, P., Zdouc, M.M., Simone, M., Maffioli, S.I., Ebright, R.H., Donadio, S., Sosio, M.“Blocks in the pseudouridimycin pathway unlock hidden metabolites in the Streptomyces producer strain”. Sci Rep. (2021)11(1):5827. doi: 10.1038/s41598-021-84833-2.
Schorn, M.A. et al. “A community resource for paired genomic and metabolomic data mining”. Nat Chem Biol. (2021) doi: 10.1038/s41589-020-00724-z.
Zdouc, M.M., Iorio, M., Maffioli, S.I., Crüsemann, M., Donadio, S., Sosio, M. “Planomonospora: A Metabolomics Perspective on an Underexplored Actinobacteria Genus”. J Nat Prod. (2021). doi: 10.1021/acs.jnatprod.0c00807.
Zdouc, M.M., Iorio, M., Vind, K., Simone, M., Serina, S., Brunati, C., Monciardini, P., Tocchetti, A., Zarazúa, G.S., Crüsemann, M., Maffioli, S.I., Sosio, M., Donadio, S. “Effective approaches to discover new microbial metabolites in a large strain library”. J Ind Microbiol Biotechnol. (2021) doi: 10.1093/jimb/kuab017.
2020
Zdouc MM, Alanjary MM, Zarazúa GS, Maffioli SI, Crüsemann M, Medema MH, Donadio S, Sosio M. A biaryl-linked tripeptide from Planomonospora reveals a widespread class of minimal RiPP gene clusters. Cell Chem Biol. 2020 doi: 10.1016/j.chembiol.2020.11.009.
Alduina R, Tocchetti A, Costa S, Ferraro C, Cancemi P, Sosio M, Donadio S. A Two-Component regulatory system with opposite effects on glycopeptide antibiotic biosynthesis and resistance. Sci Rep. 2020 Apr 10;10(1):6200. doi: 10.1038/s41598-020-63257-4.
2019
Musiol-Kroll EM, Tocchetti A, Sosio M, Stegmann E. Challenges and advances in genetic manipulation of filamentous actinomycetes - the remarkable producers of specialized metabolites. Nat Prod Rep. 2019 Sep 1;36(9):1351-1369. doi: 10.1039/c9np00029a.
Alt S, Bernasconi A, Sosio M, Brunati C, Donadio S, Maffioli SI. Toward Single-Peak Dalbavancin Analogs through Biology and Chemistry. ACS Chem Biol. 2019 Mar 15;14(3):356-360. doi: 10.1021/acschembio.9b00050.
Monciardini P, Bernasconi A, Iorio M, Brunati C, Sosio M, Campochiaro L, Landini P, Maffioli SI, Donadio S. Antibacterial Aromatic Polyketides Incorporating the Unusual Amino Acid Enduracididine. J Nat Prod. 2019 Jan 25;82(1):35-44. doi: 10.1021/acs.jnatprod.8b00354.
Maffioli SI, Sosio M, Ebright RH, Donadio S. Discovery, properties, and biosynthesis of pseudouridimycin, an antibacterial nucleoside-analog inhibitor of bacterial RNA polymerase. J Ind Microbiol Biotechnol. 2019 Mar;46(3-4):335-343. doi: 10.1007/s10295-018-2109-2.
2018
Iorio M, Tocchetti A, Santos Cruz JC, Del Gatto G, Brunati C, Maffioli SI, Sosio M, Donadio S. Novel Polyethers from Screening Actinoallomurus spp. Antibiotics (Basel). 2018 Jun 14;7(2). pii: E47. doi: 10.3390/antibiotics7020047.
Tocchetti A, Donadio S, Sosio M. Large inserts for big data: artificial chromosomes in the genomic era. FEMS Microbiol Lett. 2018 May 1;365(9). doi: 10.1093/femsle/fny064.
Alduina R, Sosio M, Donadio S. Complex Regulatory Networks Governing Production of the Glycopeptide A40926. Antibiotics (Basel). 2018 Apr 5;7(2). pii: E30. doi: 10.3390/antibiotics7020030. Review.
Sosio M, Gaspari E, Iorio M, Pessina S, Medema MH, Bernasconi A, Simone M, Maffioli SI, Ebright RH, Donadio S. Analysis of the Pseudouridimycin Biosynthetic Pathway Provides Insights into the Formation of C-nucleoside Antibiotics. Cell Chem Biol. 2018 May 17;25(5):540-549.e4. doi: 10.1016/j.chembiol.2018.02.008.
Brunati C, Thomsen TT, Gaspari E, Maffioli S, Sosio M, Jabes D, Lobner-Olesen A, Donadio S. (2018) Expanding the potential of NAI-107 for treating ESKAPE pathogens: synergistic combinations against Gram-negatives and bactericidal activity against non-dividing cells J. Antimicrob. Chemother. 73: 414-424. doi: 10.1093/jac/dkx395
2017
Maffioli SI, Zhang Y, Degen D, Carzaniga T, Del Gatto G, Serina S, Monciardini P, Mazzetti C, Guglierame P, Candiani G, Chiriac AI, Facchetti G, Kaltofen P, Sahl HG, Dehò G, Donadio S, Ebright RH. (2017) Antibacterial Nucleoside-Analog Inhibitor of Bacterial RNA Polymerase Cell 169(7): 1240-1248.e23. doi: 10.1016/j.cell.2017.05.042.
Iorio M, Cruz J, Simone M, Bernasconi A, Brunati C, Sosio M, Donadio S, Maffioli SI. Antibacterial Paramagnetic Quinones from Actinoallomurus. J Nat Prod. 2017 Apr 28;80(4):819-827. doi: 10.1021/acs.jnatprod.6b00654.
Ortega MA, Cogan DP, Mukherjee S, Garg N, Li B, Thibodeaux GN, Maffioli SI, Donadio S, Sosio M, Escano J, Smith L, Nair SK, van der Donk WA Two Flavoenzymes Catalyze the Post-Translational Generation of 5-Chlorotryptophan and 2-Aminovinyl-Cysteine during NAI-107 Biosynthesis. ACS Chem Biol. 2017 Feb 17;12(2):548-557. doi: 10.1021/acschembio.6b01031
Cruz JC, Maffioli SI, Bernasconi A, Brunati C, Gaspari E, Sosio M, Wellington E, Donadio S. Allocyclinones, hyperchlorinated angucyclinones from Actinoallomurus. J Antibiot (Tokyo). 2017 Jan;70(1):73-78. doi: 10.1038/ja.2016.62.
2016
Ortega MA, Hao Y, Walker MC, Donadio S, Sosio M, Nair SK, van der Donk WA. Structure and tRNA Specificity of MibB, a Lantibiotic Dehydratase from Actinobacteria Involved in NAI-107 Biosynthesis. Cell Chem Biol. 2016 Mar 17;23(3):370-380. doi: 10.1016/j.chembiol.2015.11.017.
Thomsen TT, Mojsoska B, Cruz JC, Donadio S, Jenssen H, Løbner-Olesen A, Rewitz K. The Lantibiotic NAI-107 Efficiently Rescues Drosophila melanogaster from Infection with Methicillin-Resistant Staphylococcus aureus USA300. Antimicrob Agents Chemother. 2016 Aug 22;60(9):5427-36. doi: 10.1128/AAC.02965-15.
Maffioli SI, Cruz JC, Monciardini P, Sosio M, Donadio S. Advancing cell wall inhibitors towards clinical applications. J Ind Microbiol Biotechnol. 2016 Mar;43(2-3):177-84. doi: 10.1007/s10295-015-1703-9.
Gallo G, Renzone G, Palazzotto E, Monciardini P, Arena S, Faddetta T, Giardina A, Alduina R, Weber T, Sangiorgi F, Russo A, Spinelli G, Sosio M, Scaloni A, Puglia AM. Elucidating the molecular physiology of lantibiotic NAI-107 production in Microbispora ATCC-PTA-5024. BMC Genomics. 2016 Jan 12;17:42. doi: 10.1186/s12864-016-2369-z.
2015
Cruz JC, Iorio M, Monciardini P, Simone M, Brunati C, Gaspari E, Maffioli SI, Wellington E, Sosio M, Donadio S. Brominated Variant of the Lantibiotic NAI-107 with Enhanced Antibacterial Potency. J Nat Prod. 2015 Nov 25;78(11):2642-7. doi: 10.1021/acs.jnatprod.5b00576.
Medema MH, Kottmann R, Yilmaz et al., Minimum Information about a Biosynthetic Gene cluster. Nat Chem Biol. 2015 Sep;11(9):625-31. doi: 10.1038/nchembio.1890
Maffioli SI, Monciardini P, Catacchio B, Mazzetti C, Munch D, Brunati C, Sahl HG, Donadio S. (2015) Family of Class I Lantibiotics from Actinomycetes and Improvement of Their Antibacterial Activities ACS Chemical Biology, 10(4): 1034-1042.
Tocchetti A, Bordoni R, Gallo G, Petiti L, Corti G, Alt S, Cruz JCS, Salzano AM, Scaloni A, Puglia AM, De Bellis G, Peano C, Donadio S, Sosio M. (2015) A Genomic, Transcriptomic and Proteomic Look at the GE2270 Producer Planobispora rosea, an Uncommon Actinomycete PLos ONE 10(7) e0133705
Fabbretti A, He CG, Gaspari E, Maffioli S, Brandi L, Spurio R, Sosio M, Jabes D, Donadio S. (2015) A derivative of the thiopeptide GE2270A highly selective against Propionibacterium acnes Antimicrobial Agents and Chemotherapy , 59(8): 4560-4568.
Lo Grasso L, Maffioli S, Sosio M, Bibb M, Puglia AM, Alduina R. Two Master Switch Regulators Trigger A40926 Biosynthesis in Nonomuraea sp. Strain ATCC 39727. J Bacteriol. 2015 Aug 1;197(15):2536-44. doi: 10.1128/JB.00262-15.
2014
Monciardini P, Iorio M, Maffioli S, Sosio M and Donadio S. (2014) Discovering new bioactive molecules from microbial sources Microbial Biotechnology, 7(3): 209-220. doi: 10.1111/1751-7915.12123.
Zhang Y, Degen D, Ho MX, Sineva E, Ebright KY, Ebright YW, Mekler V, Vahedian-Mohaved H, Feng Y, Yin R, Tuske S, Irschik H, Jansen R, Maffioli S, Donadio S, Arnold E and Ebright RH. (2014) GE23077 binds to the RNA polymerase "I" and "I+1" sites and prevents the binding of initiating nucleotides eLife, DOI: 10.7554/eLife.02450.
Iorio M, Sasso O, Maffioli SI, Bertorelli R, Monciardini P, Sosio M, Bonezzi F, Summa M, Brunati C, Bordoni R, Corti G, Tarozzo G, Piomelli D, Reggiani A, Donadio S. (2014) A glycosylated, labionin-containing lanthipeptide with marked antinociceptive activity ACS Chem. Biol. 9: 398-404. doi: 10.1021/cb400692w.
Jabes D, Brunati C, Candiani G, Riva S, Romanó G, Maffioli S, Rossi R, Simone M, Gaspari E, Donadio S. (2014) Pharmacological Properties of NAI-603, a Well-Tolerated Semisynthetic Derivative of Ramoplanin Antimicrob. Agents Chemother. 58: 1922-1929. doi: 10.1128/AAC.01620-13.
Giardina A, Alduina R, Gallo G, Monciardini P, Sosio M, Puglia AM. Inorganic phosphate is a trigger factor for Microbispora sp. ATCC-PTA-5024 growth and NAI-107 production. Microb Cell Fact. 2014 Oct 10;13:133. doi: 10.1186/s12934-014-0133-0.
Flinspach K, Kapitzke C, Tocchetti A, Sosio M, Apel AK. Heterologous expression of the thiopeptide antibiotic GE2270 from Planobispora rosea ATCC 53733 in Streptomyces coelicolor requires deletion of ribosomal genes from the expression construct. PLoS One. 2014 Mar 5;9(3):e90499. doi: 10.1371/journal.pone.0090499.
Sosio M, Gallo G, Pozzi R, Serina S, Monciardini P, Bera A, Stegmann E, Weber T. Draft Genome Sequence of the Microbispora sp. Strain ATCC-PTA-5024, Producing the Lantibiotic NAI-107. Genome Announc. 2014 Jan 23;2(1). pii: e01198-13. doi: 10.1128/genomeA.01198-13.
Maffioli SI, Iorio M, Sosio M, Monciardini P, Gaspari E, Donadio S. Characterization of the congeners in the lantibiotic NAI-107 complex. J Nat Prod. 2014 Jan 24;77(1):79-84. doi: 10.1021/np400702t.
Münch D, Müller A, Schneider T, Kohl B, Wenzel M, Bandow JE, Maffioli S, Sosio M, Donadio S, Wimmer R. Sahl HG.(2014) The lantibiotic NAI-107 binds to bactoprenol bound cell wall precursors and impairs membrane functions J. Biol. Chem. 289(17): 12063-76. doi: 10.1074/jbc.M113.537449.
