Recent Publications


Alduina R, Tocchetti A, Costa S, Ferraro C, Cancemi P, Sosio M, Donadio S. A Two-Component regulatory system with opposite effects on glycopeptide antibiotic biosynthesis and resistance. Sci Rep. 2020 Apr 10;10(1):6200. doi: 10.1038/s41598-020-63257-4.



Musiol-Kroll EM, Tocchetti A, Sosio M, Stegmann E. Challenges and advances in genetic manipulation of filamentous actinomycetes - the remarkable producers of specialized metabolites. Nat Prod Rep. 2019 Sep 1;36(9):1351-1369. doi: 10.1039/c9np00029a.

Alt S, Bernasconi A, Sosio M, Brunati C, Donadio S, Maffioli SI. Toward Single-Peak Dalbavancin Analogs through Biology and Chemistry. ACS Chem Biol. 2019 Mar 15;14(3):356-360. doi: 10.1021/acschembio.9b00050.

Monciardini P, Bernasconi A, Iorio M, Brunati C, Sosio M, Campochiaro L, Landini P, Maffioli SI, Donadio S. Antibacterial Aromatic Polyketides Incorporating the Unusual Amino Acid Enduracididine. J Nat Prod. 2019 Jan 25;82(1):35-44. doi: 10.1021/acs.jnatprod.8b00354.

Maffioli SI, Sosio M, Ebright RH, Donadio S. Discovery, properties, and biosynthesis of pseudouridimycin, an antibacterial nucleoside-analog inhibitor of bacterial RNA polymerase. J Ind Microbiol Biotechnol. 2019 Mar;46(3-4):335-343. doi: 10.1007/s10295-018-2109-2.



Iorio M, Tocchetti A, Santos Cruz JC, Del Gatto G, Brunati C, Maffioli SI, Sosio M, Donadio S. Novel Polyethers from Screening Actinoallomurus spp. Antibiotics (Basel). 2018 Jun 14;7(2). pii: E47. doi: 10.3390/antibiotics7020047.

Tocchetti A, Donadio S, Sosio M. Large inserts for big data: artificial chromosomes in the genomic era. FEMS Microbiol Lett. 2018 May 1;365(9). doi: 10.1093/femsle/fny064.

Alduina R, Sosio M, Donadio S. Complex Regulatory Networks Governing Production of the Glycopeptide A40926. Antibiotics (Basel). 2018 Apr 5;7(2). pii: E30. doi: 10.3390/antibiotics7020030. Review.

Sosio M, Gaspari E, Iorio M, Pessina S, Medema MH, Bernasconi A, Simone M, Maffioli SI, Ebright RH, Donadio S. Analysis of the Pseudouridimycin Biosynthetic Pathway Provides Insights into the Formation of C-nucleoside Antibiotics. Cell Chem Biol. 2018 May 17;25(5):540-549.e4. doi: 10.1016/j.chembiol.2018.02.008.

Brunati C, Thomsen TT, Gaspari E, Maffioli S, Sosio M, Jabes D, Lobner-Olesen A, Donadio S. (2018) Expanding the potential of NAI-107 for treating ESKAPE pathogens: synergistic combinations against Gram-negatives and bactericidal activity against non-dividing cells J. Antimicrob. Chemother. 73: 414-424. doi: 10.1093/jac/dkx395



Maffioli SI, Zhang Y, Degen D, Carzaniga T, Del Gatto G, Serina S, Monciardini P, Mazzetti C, Guglierame P, Candiani G, Chiriac AI, Facchetti G, Kaltofen P, Sahl HG, Dehò G, Donadio S, Ebright RH. (2017) Antibacterial Nucleoside-Analog Inhibitor of Bacterial RNA Polymerase Cell 169(7): 1240-1248.e23. doi: 10.1016/j.cell.2017.05.042.

Iorio M, Cruz J, Simone M, Bernasconi A, Brunati C, Sosio M, Donadio S, Maffioli SI. Antibacterial Paramagnetic Quinones from Actinoallomurus. J Nat Prod. 2017 Apr 28;80(4):819-827. doi: 10.1021/acs.jnatprod.6b00654.

Ortega MA, Cogan DP, Mukherjee S, Garg N, Li B, Thibodeaux GN, Maffioli SI, Donadio S, Sosio M, Escano J, Smith L, Nair SK, van der Donk WA Two Flavoenzymes Catalyze the Post-Translational Generation of 5-Chlorotryptophan and 2-Aminovinyl-Cysteine during NAI-107 Biosynthesis. ACS Chem Biol. 2017 Feb 17;12(2):548-557. doi: 10.1021/acschembio.6b01031

Cruz JC, Maffioli SI, Bernasconi A, Brunati C, Gaspari E, Sosio M, Wellington E, Donadio S. Allocyclinones, hyperchlorinated angucyclinones from Actinoallomurus. J Antibiot (Tokyo). 2017 Jan;70(1):73-78. doi: 10.1038/ja.2016.62.



Ortega MA, Hao Y, Walker MC, Donadio S, Sosio M, Nair SK, van der Donk WA. Structure and tRNA Specificity of MibB, a Lantibiotic Dehydratase from Actinobacteria Involved in NAI-107 Biosynthesis. Cell Chem Biol. 2016 Mar 17;23(3):370-380. doi: 10.1016/j.chembiol.2015.11.017.

Thomsen TT, Mojsoska B, Cruz JC, Donadio S, Jenssen H, Løbner-Olesen A, Rewitz K. The Lantibiotic NAI-107 Efficiently Rescues Drosophila melanogaster from Infection with Methicillin-Resistant Staphylococcus aureus USA300. Antimicrob Agents Chemother. 2016 Aug 22;60(9):5427-36. doi: 10.1128/AAC.02965-15.

Maffioli SI, Cruz JC, Monciardini P, Sosio M, Donadio S. Advancing cell wall inhibitors towards clinical applications. J Ind Microbiol Biotechnol. 2016 Mar;43(2-3):177-84. doi: 10.1007/s10295-015-1703-9.

Gallo G, Renzone G, Palazzotto E, Monciardini P, Arena S, Faddetta T, Giardina A, Alduina R, Weber T, Sangiorgi F, Russo A, Spinelli G, Sosio M, Scaloni A, Puglia AM. Elucidating the molecular physiology of lantibiotic NAI-107 production in Microbispora ATCC-PTA-5024. BMC Genomics. 2016 Jan 12;17:42. doi: 10.1186/s12864-016-2369-z.



Cruz JC, Iorio M, Monciardini P, Simone M, Brunati C, Gaspari E, Maffioli SI, Wellington E, Sosio M, Donadio S. Brominated Variant of the Lantibiotic NAI-107 with Enhanced Antibacterial Potency. J Nat Prod. 2015 Nov 25;78(11):2642-7. doi: 10.1021/acs.jnatprod.5b00576.

Medema MH, Kottmann R, Yilmaz et al., Minimum Information about a Biosynthetic Gene cluster. Nat Chem Biol. 2015 Sep;11(9):625-31. doi: 10.1038/nchembio.1890

Maffioli SI, Monciardini P, Catacchio B, Mazzetti C, Munch D, Brunati C, Sahl HG, Donadio S. (2015) Family of Class I Lantibiotics from Actinomycetes and Improvement of Their Antibacterial Activities ACS Chemical Biology, 10(4): 1034-1042.

Tocchetti A, Bordoni R, Gallo G, Petiti L, Corti G, Alt S, Cruz JCS, Salzano AM, Scaloni A, Puglia AM, De Bellis G, Peano C, Donadio S, Sosio M. (2015) A Genomic, Transcriptomic and Proteomic Look at the GE2270 Producer Planobispora rosea, an Uncommon Actinomycete PLos ONE 10(7) e0133705

Fabbretti A, He CG, Gaspari E, Maffioli S, Brandi L, Spurio R, Sosio M, Jabes D, Donadio S. (2015) A derivative of the thiopeptide GE2270A highly selective against Propionibacterium acnes Antimicrobial Agents and Chemotherapy , 59(8): 4560-4568.

Lo Grasso L, Maffioli S, Sosio M, Bibb M, Puglia AM, Alduina R. Two Master Switch Regulators Trigger A40926 Biosynthesis in Nonomuraea sp. Strain ATCC 39727. J Bacteriol. 2015 Aug 1;197(15):2536-44. doi: 10.1128/JB.00262-15.



Monciardini P, Iorio M, Maffioli S, Sosio M and Donadio S. (2014) Discovering new bioactive molecules from microbial sources Microbial Biotechnology, 7(3): 209-220. doi: 10.1111/1751-7915.12123.

Zhang Y, Degen D, Ho MX, Sineva E, Ebright KY, Ebright YW, Mekler V, Vahedian-Mohaved H, Feng Y, Yin R, Tuske S, Irschik H, Jansen R, Maffioli S, Donadio S, Arnold E and Ebright RH. (2014) GE23077 binds to the RNA polymerase "I" and "I+1" sites and prevents the binding of initiating nucleotides eLife, DOI: 10.7554/eLife.02450.

Iorio M, Sasso O, Maffioli SI, Bertorelli R, Monciardini P, Sosio M, Bonezzi F, Summa M, Brunati C, Bordoni R, Corti G, Tarozzo G, Piomelli D, Reggiani A, Donadio S. (2014) A glycosylated, labionin-containing lanthipeptide with marked antinociceptive activity ACS Chem. Biol. 9: 398-404. doi: 10.1021/cb400692w.

Jabes D, Brunati C, Candiani G, Riva S, Romanó G, Maffioli S, Rossi R, Simone M, Gaspari E, Donadio S. (2014) Pharmacological Properties of NAI-603, a Well-Tolerated Semisynthetic Derivative of Ramoplanin Antimicrob. Agents Chemother. 58: 1922-1929. doi: 10.1128/AAC.01620-13.

Giardina A, Alduina R, Gallo G, Monciardini P, Sosio M, Puglia AM. Inorganic phosphate is a trigger factor for Microbispora sp. ATCC-PTA-5024 growth and NAI-107 production. Microb Cell Fact. 2014 Oct 10;13:133. doi: 10.1186/s12934-014-0133-0.

Flinspach K, Kapitzke C, Tocchetti A, Sosio M, Apel AK. Heterologous expression of the thiopeptide antibiotic GE2270 from Planobispora rosea ATCC 53733 in Streptomyces coelicolor requires deletion of ribosomal genes from the expression construct. PLoS One. 2014 Mar 5;9(3):e90499. doi: 10.1371/journal.pone.0090499.

Sosio M, Gallo G, Pozzi R, Serina S, Monciardini P, Bera A, Stegmann E, Weber T. Draft Genome Sequence of the Microbispora sp. Strain ATCC-PTA-5024, Producing the Lantibiotic NAI-107. Genome Announc. 2014 Jan 23;2(1). pii: e01198-13. doi: 10.1128/genomeA.01198-13.

Maffioli SI, Iorio M, Sosio M, Monciardini P, Gaspari E, Donadio S. Characterization of the congeners in the lantibiotic NAI-107 complex. J Nat Prod. 2014 Jan 24;77(1):79-84. doi: 10.1021/np400702t.

Münch D, Müller A, Schneider T, Kohl B, Wenzel M, Bandow JE, Maffioli S, Sosio M, Donadio S, Wimmer R. Sahl HG.(2014) The lantibiotic NAI-107 binds to bactoprenol bound cell wall precursors and impairs membrane functions J. Biol. Chem. 289(17): 12063-76. doi: 10.1074/jbc.M113.537449.